The purpose of the present study was to determine whether or not muscle insulin resistance induced by a high-fat diet is rapidly reversed. After induction of insulin resistance by triglycerides (TG) and heparin to raise free fatty acids (FFA), a washout incubation of muscles in vitro rapidly reversed insulin resistance. However, in muscles from rats fed with high-fat diet for 5 weeks incubated in absence of FFA, the insulin resistance was maintained and even a 310 minutes washout incubation of muscles in vitro had no effect. Thus, these results show the existence of two different types of fat-induced insulin resistance. The authors question the use of raising FFA levels with the heparin model in order to study the mechanism responsible for insulin resistance in patients with the metabolic syndrome and type 2 diabetes.
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