Adiponectin protects against angiotensin II or tumor necrosis factor alpha-induced endothelial cell monolayer hyperpermeability: role of cAMP/PKA signaling.

Description

Xu et al. tested the hypothesis that adiponectin protects against hyperpermeability of an endothelial cell monolayer induced by angiotensin II (Ang II) or tumor necrosis factor (TNF)-a levels. They also evaluated the potential role of the cAMP/PKA signalling cascade in adiponectin suppression of endothelial permeability assessed by measuring transendothelial electric resistance (TEER). This study demonstrated that adiponectin protects against increased permeability of an endothelial cell monolayer induced by TNF-a or Ang II in a cAMP/PKA-dependent manner. The authors found that adiponectin suppressed multiple cellular effects known to be associated with endothelial hyperpermeability, including actin stress fibre development, intercellular gap formation, and β-tubulin disassembly. Both the full-length form of adiponectin as well as the recombinant globular form protected endothelial barrier integrity. These findings highlight the cAMP/PKA pathway as a major signalling system that mediates the effects of adiponectin in endothelial cells.