The cross-sectional associations between lifestyle factors and abdominal subcutaneous and intra-abdominal (visceral) adipose tissue were assessed in 2,926 Framingham Heart Study participants. Adherence to recommended dietary guidelines and physical activity were inversely related to subcutaneous and intra-abdominal adipose tissue accumulation. However, smoking and alcohol intake were differentially associated with intra-abdominal adipose tissue. Finally, an increasing number of healthy lifestyle factors were associated with lower subcutaneous and intra-abdominal adipose tissue.